Rapid isocratic HPLC investigation of radiochemical purity for Y-90-DOTATATE

Abstract

DOTA-conjugated peptides, such as [DOTA(0), Tyr(3)]ocreotide (DOTATOC) and [DOTA(0), Tyr(3)]octreotate (DOTATATE) can be labeled with radionuclides such as Y-90, Lu-177 and In-111 at high specific activities. These radiolabelled somatostatin analogues are used for peptide receptor radionuclide therapy (PRRT). Currently, radio-high performance liquid chromatography (radio-HPLC) and radio-thin layer chromatography (radio-TLC) are the methods of choice for the analysis of the labeled compounds. In literature, radiochemical purity of the radiolabelled DOTATATE was investigated using gradient reversed-phase radio-HPLC. However, these studies indicate long retention time of the radiolabelled compound of 14.52 min. In our study, a new simple and rapid reversed-phase isocratic system enables the radiochemical purity of the radiolabelled DOTATATE within a few minutes.