Selection of biomarker from candidate miRNAs for early detection of ovarian cancer

Abstract

Ovarian Ovarian cancer cancercancer (OC)(OC) is one of the most most common common gynaecological gynaecological malignancies malignancies malignanciesamong among womenwomen women.In ovarian malignancies malignancies malignancies,approximately approximately approximately approximately approximately90 %of cases cases are epithelial epithelial ovarian cancer cancer(EOC), (EOC), which which is the most lethal ovarianovarian cancer cancercancer type type becausebecause becausebecause of alack of screening screening screening tests and asymptomatic asymptomatic asymptomatic asymptomatic [1].Early detection detection of EOC has agreat great importance importancein the clinic [2]. Since observation observation of cancer cancer massmass and andbiopsy biopsyareare difficult difficult in early early stages stages ,diagnosis by non -invasiveinvasive methods methods areare becomingbecoming becoming more more important important .Therefore Therefore ThereforeTherefore Therefore ,the thedetectiondetection detection of tumor tumor tumormaterials materials materials passed to body body fluids in the first stage stage of cancer cancercancer can make possible possible possible possiblethe early early diagnosis diagnosisdiagnosis of cancer cancercancer .MicroRNAs MicroRNAs (miRNAs miRNAs ), oneone type type of specific specificspecific biomarkers biomarkersbiomarkers forfor the pathogenesis pathogenesis pathogenesis pathogenesis of cancer cancercancer ,areare usedused for fordetection detection detection detection,prognosis prognosis prognosis ,diagnosis diagnosis diagnosis ,and therapy therapyin several several diseases diseases diseases [3]. The purpose purposepurpose purpose of this study was wasto identify identify identifynovel circulating circulating miRNAs miRNAsmiRNAsto be usedused usedas early early diagnosis diagnosis diagnosis andand predictionprediction prediction prediction toolstools forfor EOC .Detection Detection Detection of thethe differences differences differences differencesdifferences in miRNAs miRNAs expression expression havehave been validated validated by RT -qPCR .Different Different Different expressions expressions expressions expressions of hsa -miR -19091909 -5p, hsa hsa-miR -885885 -5p,and andhsa -letlet -7d-3pwerewere statistically statistically statistically significant significant (p <0.05 ).It has been detecteddetected detected detectedwhich which genesgenes genesand related related related pathways pathwayspathwaysaffected affected by the validated validated miRNAs miRNAs using using "Pathway "Pathway Studio" Studio" database database.