Effects of Different Parthenogenetic Activation Periods on Mouse Embryo Development and Quality

Abstract

In the present study we investigate the effects of parthenogenetic activation on in vitro embryo development and quality in different activation periods. oocytes were obtained 14 hours after human chorionic gonadotropin (hCG) injection from superovulated B6D2F1 female mice then parthenogenetic activation started 18 hours after hCG injection. The oocytes were activated at different activation periods for 3, 4, 5 or 6 hours in 10 mM strontium chloride (SrCl2)+ 5 μg/mL-1 Cytohalasine B (CB) + 5 nM Trichostatin A (TSA) containing a Ca 2+ free Chatot Ziomek Brinster (CZB) activation medium, followed by further incubation for two hours at 37°C and 5% CO2 in embryo culturing medium + TSA. The results in the present study suggested that the parthenogenetic activation of the 6 hour activation period was found to be higher than at 3, 4 and 5 hours.