Flow Cytometric Evaluation of Pregnancy-Induced Anti-Donor Immunization

Abstract

Background. Living donor kidneys from spouses and children (from offspring to parents) are currently considered to be important organ sources. However, pregnancy-induced alloimmunization may provoke acute rejection episodes after kidney transplantation, being flow cytometry cross-match (FCXM) we studied donor-specific antibodies (DSAs) in the sera of recipients planned for living kidney transplantation from their spouse or children. When the FCXM was positive, we confirmed the existence of anti-human leukocyte antigen (H LA) antibodies using flow cytometry panel-reactive antibody (flow-PRA).