Development of a Rapid Molecular Test Format Based on Isothermal Recombinase Polymerase Amplification Technique and Lateral Flow Method for Detection of bla(OXA-48)

Abstract

Objective: Molecular tests are rapid, reliable tools for the detection of carbapenem resistance, but their use is limited due to their cost, requirement for well-trained technicians and highly sophisticated instrumentation. The recombinase polymerase amplification (RPA) assay, one of the isothermal amplification methods developed recently to overcome these problems, doesn't require denaturation of target, and RPA products can be determined by probe-base detection methods even without a specific instrumentation. In this study, we aimed to develop a rapid and easily applicable molecular test format in on-site settings based on RPA technique with combination of lateral flow system for detection of bla(OXA-48).