Polymeric nanoparticles for selective protein recognition by using thiol-ene miniemulsion photopolymerization
Yazar
Aktas, Zerrin
KAHRAMAN, MEMET VEZİR
KAYAMAN APOHAN, NİLHAN
Akcakaya, Handan
Oncul, Mustafa Oral
Yasar, Merve
Dal Yontem, Fulya
Üst veri
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The fabrication of molecularly imprinted nanoparticles (MIP-NPs) specific for myoglobin by using thiol-ene photopolymerization in miniemulsion was described. Allyl derivatives of phenylalanine as a functional monomer was synthesized and copolymerized with acrylic monomersviaminiemulsion polymerization to produce NIP-NPs with approximately 74 nm number average particle diameter. FTIR and(1)H-NMR analysis confirmed the synthesis of functional monomer. MIP-NPs were prepared in the existence of myoglobin as a template protein. Morphological investigations exhibited that the particle size of the MIP-NPs, increased compared to the corresponding NIPs and the mean particle diameter by number was measured as 141 nm with narrow distribution. NIP-NPs that were polymerized without myoglobin were found to have less affinity to the target protein. In addition, the rebinding ability of MIP-NPs was much bigger than that of the corresponding NIPs. ELISA results showed that MIPs interact particularly with the myoglobin and show little affinity for BSA in competitive binding experiments.
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- Makale [92796]