Effects of Crataegus microphylla on Vascular Dysfunction in Streptozotocin-induced Diabetic Rats
Tarih
2013Yazar
Demirci, Cihan
Topal, Gökce
Melikoglu, Gülay
Dogan, B. Sonmez Uydes
Kucur, Mine
Koc, Ebru
Meriçli, Ali H.
Ozdemir, Osman
Ergin, Bulent
Altug, Tuncay
Karaca, Cetin
Üst veri
Tüm öğe kaydını gösterÖzet
Vascular dysfunction plays a key role in the pathogenesis of diabetic vascular disease. In this study, we aimed to investigate whether chronic in vivo treatment of Crataegus microphylla (CM) extract in diabetic rats induced with streptozotocin (STZ, intraperitoneal, 65mg/kg) preserves vascular function and to evaluate whether the reduction of inducible nitric oxide synthase (iNOS), proinflammatory cytokines, and lipid peroxidation mediates its mechanisms of action. Starting at 4weeks of diabetes, CM extract (100mg/kg) was administrated to diabetic rats for 4weeks. In aortic rings, relaxation to acetylcholine and vasoreactivity to noradrenaline were impaired, whereas aortic iNOS expression and plasma tumor necrosis factor- (TNF-) and interleukin-6 (IL-6), total nitritenitrate, and malondialdehite levels were increased in diabetic rats compared with controls. Chronic CM treatment significantly corrected all the above abnormalities in diabetic rats. In comparison, pretreatment of the aorta of diabetic rats with N-[3(aminomethyl) benzyl]-acetamidine, dihydrochloride (105M), a selective inhibitor of iNOS, produced a similar recovery in vascular reactivity. These results suggest that chronic in vivo treatment of CM preserves endothelium-dependent relaxation and vascular contraction in STZ-induced diabetes, possibly by reducing iNOS expression in the aorta and by decreasing plasma levels of TNF- and IL-6 and by preventing lipid peroxidation. Copyright (c) 2012 John Wiley & Sons, Ltd.
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